High-speed atomic force microscopy of protein dynamics: myosin on actin and rotary enzyme F1-ATPase
نویسنده
چکیده
AFM allows the visualization of biological samples under physiological solution conditions, at high spatial resolution. However, captured images are limited to snapshots because it takes at least 30 seconds to capture an image, whereas biological phenomena are highly dynamic. To overcome this limitation, my group has developed high-speed AFM. The dynamic processes and structural dynamics of protein molecules in action can now be visualized using HS-AFM. Here we describe our experimental set-up for HS-AFM and report on its application to the study of two biological mechanotransducer systems: the movement of myosin V on actin filaments and the conformational changes in rotorless F1-ATPase.
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